Journal: BMC Biotechnology

Article Title: Evaluation of five different cDNA labeling methods for microarrays using spike controls

doi: 10.1186/1472-6750-3-23

Figure Lengend Snippet: Accuracy and reproducibility of five different labeling methods in cDNA microarray hybridizations 1 .

Article Snippet: For the CyScribe, FairPlay and TSA Micromax methods, the hybridization-mixture was slightly modified compared to manufacturer's recommended procedure: 7.9 μl 20x SSC, 1.4 μl 10% SDS, 2.5 μl 50x Denhardt, 1 μl yeast tRNA (4 μg/μl), 1 μl poly d(A) (6 μg/μl) and 2 μl 1% BSA were added to the labeled and purified cDNA.

Techniques: Labeling, Microarray

Journal: Scientific Reports

Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

doi: 10.1038/s41598-021-97559-y

Figure Lengend Snippet: The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

Article Snippet: The sensitivity and specificity obtained in this study are similar to the results obtained in a previous systematic review of the CapitalBio DNA microarray method .

Techniques: Microarray, Control, Negative Control

Journal: Scientific Reports

Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

doi: 10.1038/s41598-021-97559-y

Figure Lengend Snippet: The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

Article Snippet: The sensitivity and specificity obtained in this study are similar to the results obtained in a previous systematic review of the CapitalBio DNA microarray method .

Techniques: Microarray

Journal: Scientific Reports

Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

doi: 10.1038/s41598-021-97559-y

Figure Lengend Snippet: Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

Article Snippet: The sensitivity and specificity obtained in this study are similar to the results obtained in a previous systematic review of the CapitalBio DNA microarray method .

Techniques: Comparison, Diagnostic Assay, Microarray

Journal: Scientific Reports

Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

doi: 10.1038/s41598-021-97559-y

Figure Lengend Snippet: Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

Article Snippet: The sensitivity and specificity obtained in this study are similar to the results obtained in a previous systematic review of the CapitalBio DNA microarray method .

Techniques: Microarray

Journal: Scientific Reports

Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

doi: 10.1038/s41598-021-97559-y

Figure Lengend Snippet: Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

Article Snippet: The sensitivity and specificity obtained in this study are similar to the results obtained in a previous systematic review of the CapitalBio DNA microarray method .

Techniques: Microarray, Mutagenesis